Food that is lost before it reaches the consumer, and food that is wasted by consumers, has been estimated to account for as much as 40% of the total food produced in the United States (Buzby, Wells, and Hyman, 2014; Hall et al., 2009). This represents losses of important resources—including water, chemical inputs, and labor—as well as unused nutrients for consumers. Stakeholders along the supply chain are increasingly interested in developing improved approaches to measuring food waste, understanding its determinants, and devising strategies to ultimately reduce it. To date, a majority of food waste studies have focused on household-level waste; fewer studies have examined waste in food distribution and retail settings, and very little work has been conducted to understand the economic causes and consequences of food loss at the farm level. This Choices theme presents a collection of articles that explore food loss and food waste in the context of the U.S. food supply chain. The behavior and incentives of a variety of food system stakeholders including producers, market intermediaries (including retailers), and consumers are considered. The articles are organized along the supply chain, beginning with upstream issues of food loss proceeding through downstream topics such as household decisions concerning when to discard food. Taken together, this collection offers intriguing insights into current frontiers of the myriad private and public efforts to better characterize, quantify, and reduce food waste.